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primary antibodies targeting hif2a  (Novus Biologicals)


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    Novus Biologicals primary antibodies targeting hif2a
    ( A ) Schematic diagram of vadadustat treatment in HIF reporter ODD-luc mice. ( B ) Representative ex vivo bioluminescence imaging of luciferase activity using IVIS imager at 2 hours after vadadustat i.p. injection. ( C ) Quantification of bioluminescence intensity at 2 hours and 4 hours after vadadustat i.p. injection. Data are presented as mean ± SD. ( D ) Schematic diagram of vadadustat treatment in C57BL/6 mice. ( E ) Hif1A or <t>Hif2A</t> immunoblotting was performed on protein isolated from whole lung tissue after treatment with vadadustat. Each column represents 1 animal. ( F and G ) Quantification of Hif1a and Hif2a protein after treatment with vadadustat for 3 days. Data are presented as mean ± SD. ( H ) Schematic diagram of WA1 infection (280 PFU) in K18-hACE2 mice treated with vadadustat. ( I ) Kaplan-Meier plots of K18-hACE2 mice with vehicle or vadadustat treatment. P values were calculated with the Mantel-Cox test. ( J ) Blinded histological injury scores of the lungs were quantified as described in the Methods. Data are represented as mean ± SEM. ( K ) Representative H&E staining images of lung tissue from vehicle- and vadadustat-treated mice. Scale bars: 50 μm. ( L ) Schematic diagram of MA10 infection (200 PFU) in BALB/c mice treated with vadadustat. ( M ) Kaplan-Meier plots of BALB/c mice with vehicle or vadadustat treatment. P values were calculated with the Mantel-Cox test. * P < 0.05, ** P < 0.01, *** P < 0.001 by 1-way ANOVA with Dunnett’s multiple-comparison test ( C ) or 2-tailed Student’s t test ( F , G , and J ).
    Primary Antibodies Targeting Hif2a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 569 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+targeting+hif2a/pmc12288972-172-5-11?v=Novus+Biologicals
    Average 96 stars, based on 569 article reviews
    primary antibodies targeting hif2a - by Bioz Stars, 2026-07
    96/100 stars

    Images

    1) Product Images from "Identification of HIF1A as a therapeutic target during SARS-CoV-2–associated lung injury"

    Article Title: Identification of HIF1A as a therapeutic target during SARS-CoV-2–associated lung injury

    Journal: JCI Insight

    doi: 10.1172/jci.insight.191463

    ( A ) Schematic diagram of vadadustat treatment in HIF reporter ODD-luc mice. ( B ) Representative ex vivo bioluminescence imaging of luciferase activity using IVIS imager at 2 hours after vadadustat i.p. injection. ( C ) Quantification of bioluminescence intensity at 2 hours and 4 hours after vadadustat i.p. injection. Data are presented as mean ± SD. ( D ) Schematic diagram of vadadustat treatment in C57BL/6 mice. ( E ) Hif1A or Hif2A immunoblotting was performed on protein isolated from whole lung tissue after treatment with vadadustat. Each column represents 1 animal. ( F and G ) Quantification of Hif1a and Hif2a protein after treatment with vadadustat for 3 days. Data are presented as mean ± SD. ( H ) Schematic diagram of WA1 infection (280 PFU) in K18-hACE2 mice treated with vadadustat. ( I ) Kaplan-Meier plots of K18-hACE2 mice with vehicle or vadadustat treatment. P values were calculated with the Mantel-Cox test. ( J ) Blinded histological injury scores of the lungs were quantified as described in the Methods. Data are represented as mean ± SEM. ( K ) Representative H&E staining images of lung tissue from vehicle- and vadadustat-treated mice. Scale bars: 50 μm. ( L ) Schematic diagram of MA10 infection (200 PFU) in BALB/c mice treated with vadadustat. ( M ) Kaplan-Meier plots of BALB/c mice with vehicle or vadadustat treatment. P values were calculated with the Mantel-Cox test. * P < 0.05, ** P < 0.01, *** P < 0.001 by 1-way ANOVA with Dunnett’s multiple-comparison test ( C ) or 2-tailed Student’s t test ( F , G , and J ).
    Figure Legend Snippet: ( A ) Schematic diagram of vadadustat treatment in HIF reporter ODD-luc mice. ( B ) Representative ex vivo bioluminescence imaging of luciferase activity using IVIS imager at 2 hours after vadadustat i.p. injection. ( C ) Quantification of bioluminescence intensity at 2 hours and 4 hours after vadadustat i.p. injection. Data are presented as mean ± SD. ( D ) Schematic diagram of vadadustat treatment in C57BL/6 mice. ( E ) Hif1A or Hif2A immunoblotting was performed on protein isolated from whole lung tissue after treatment with vadadustat. Each column represents 1 animal. ( F and G ) Quantification of Hif1a and Hif2a protein after treatment with vadadustat for 3 days. Data are presented as mean ± SD. ( H ) Schematic diagram of WA1 infection (280 PFU) in K18-hACE2 mice treated with vadadustat. ( I ) Kaplan-Meier plots of K18-hACE2 mice with vehicle or vadadustat treatment. P values were calculated with the Mantel-Cox test. ( J ) Blinded histological injury scores of the lungs were quantified as described in the Methods. Data are represented as mean ± SEM. ( K ) Representative H&E staining images of lung tissue from vehicle- and vadadustat-treated mice. Scale bars: 50 μm. ( L ) Schematic diagram of MA10 infection (200 PFU) in BALB/c mice treated with vadadustat. ( M ) Kaplan-Meier plots of BALB/c mice with vehicle or vadadustat treatment. P values were calculated with the Mantel-Cox test. * P < 0.05, ** P < 0.01, *** P < 0.001 by 1-way ANOVA with Dunnett’s multiple-comparison test ( C ) or 2-tailed Student’s t test ( F , G , and J ).

    Techniques Used: Ex Vivo, Imaging, Luciferase, Activity Assay, Injection, Western Blot, Isolation, Infection, Staining, Comparison

    ( A ) Mice were inoculated with 3 × 10 4 PFU of the murine-adapted SARS-CoV-2 strain (MA10) via oropharyngeal aspiration, and clinical outcomes were monitored over 7 days. ( B and C ) The survival rate in SARS-CoV-2–infected mice with whole-body deletion of Hif1a ( Hif1a fl/fl UBCCreER) or ( C ) Hif2a-deleted mice ( Hif2a fl/fl UBCCreER) compared to their respective Hif fl/fl litter mates. P values were obtained using the Mantel-Cox test. ( D ) Mice with a specific deletion of Hif1a in alveolar epithelial cells ( Hif1a fl/fl SPCCreER) and their Cre-inducible counterpart (SPCCreER) were infected with 3 × 10 3 PFU of the MA10 strain via oropharyngeal aspiration or mock infected, monitored for clinical outcomes and euthanized on day 4 to harvest BALF and lung tissue. ( E ) Albumin concentration in BALF was measured by ELISA. Data are represented as mean ± SEM. Two-tailed Student’s t test. ( F and G ) Viral load in BALF and lung tissue was detected by plaque assay. Gaussian distribution was assayed using the Shapiro-Wilk test. Unpaired 2-tailed Student’s t test or Mann-Whitney U test was applied to parametric or nonparametric data, respectively. ( H ) The lungs of infected SPCCreER and Hif1a fl/fl SPCCreER mice 4 days after infection were collected, fixed, and paraffin embedded. H&E staining was performed, and images were taken at ×10 magnification ( n = 5 or 8, respectively; representative images are shown). Scale bars: 200 μm. ( I ) The lung injury score was performed blindly. In the bar-and-whisker plots, the bounds of the boxes represent the 25%–75% interquartile range, the lines within the boxes represent the median, the whiskers represent data min/max, and there are no outlying values. Two-tailed Student’s t test. ( J ) Inflammatory molecules were measured using a multiplex array in the BALF from SPCCreER and Hif1a fl/fl SPCCreER SARS-CoV-2– or mock-infected mice. Volcano plot resulting from an unpaired 2-tailed Student’s t test with Welch’s correction comparing both groups. Molecules that were highly differentially secreted are emphasized in red. The column graphs represent individual results for IL-6, G-CSF, and IP-10 ( n = 10–12). Unpaired 2-tailed Student’s t tests with Welch’s correction or Mann-Whitney U test was applied to parametric or nonparametric data. Normality was established using the Shapiro-Wilk test. * P < 0.05, ** P < 0.01, *** P < 0.001.
    Figure Legend Snippet: ( A ) Mice were inoculated with 3 × 10 4 PFU of the murine-adapted SARS-CoV-2 strain (MA10) via oropharyngeal aspiration, and clinical outcomes were monitored over 7 days. ( B and C ) The survival rate in SARS-CoV-2–infected mice with whole-body deletion of Hif1a ( Hif1a fl/fl UBCCreER) or ( C ) Hif2a-deleted mice ( Hif2a fl/fl UBCCreER) compared to their respective Hif fl/fl litter mates. P values were obtained using the Mantel-Cox test. ( D ) Mice with a specific deletion of Hif1a in alveolar epithelial cells ( Hif1a fl/fl SPCCreER) and their Cre-inducible counterpart (SPCCreER) were infected with 3 × 10 3 PFU of the MA10 strain via oropharyngeal aspiration or mock infected, monitored for clinical outcomes and euthanized on day 4 to harvest BALF and lung tissue. ( E ) Albumin concentration in BALF was measured by ELISA. Data are represented as mean ± SEM. Two-tailed Student’s t test. ( F and G ) Viral load in BALF and lung tissue was detected by plaque assay. Gaussian distribution was assayed using the Shapiro-Wilk test. Unpaired 2-tailed Student’s t test or Mann-Whitney U test was applied to parametric or nonparametric data, respectively. ( H ) The lungs of infected SPCCreER and Hif1a fl/fl SPCCreER mice 4 days after infection were collected, fixed, and paraffin embedded. H&E staining was performed, and images were taken at ×10 magnification ( n = 5 or 8, respectively; representative images are shown). Scale bars: 200 μm. ( I ) The lung injury score was performed blindly. In the bar-and-whisker plots, the bounds of the boxes represent the 25%–75% interquartile range, the lines within the boxes represent the median, the whiskers represent data min/max, and there are no outlying values. Two-tailed Student’s t test. ( J ) Inflammatory molecules were measured using a multiplex array in the BALF from SPCCreER and Hif1a fl/fl SPCCreER SARS-CoV-2– or mock-infected mice. Volcano plot resulting from an unpaired 2-tailed Student’s t test with Welch’s correction comparing both groups. Molecules that were highly differentially secreted are emphasized in red. The column graphs represent individual results for IL-6, G-CSF, and IP-10 ( n = 10–12). Unpaired 2-tailed Student’s t tests with Welch’s correction or Mann-Whitney U test was applied to parametric or nonparametric data. Normality was established using the Shapiro-Wilk test. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Techniques Used: Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Plaque Assay, MANN-WHITNEY, Staining, Whisker Assay, Multiplex Assay



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    primary antibodies targeting hif2a  (Novus Biologicals)
    96
    Novus Biologicals primary antibodies targeting hif2a
    ( A ) Schematic diagram of vadadustat treatment in HIF reporter ODD-luc mice. ( B ) Representative ex vivo bioluminescence imaging of luciferase activity using IVIS imager at 2 hours after vadadustat i.p. injection. ( C ) Quantification of bioluminescence intensity at 2 hours and 4 hours after vadadustat i.p. injection. Data are presented as mean ± SD. ( D ) Schematic diagram of vadadustat treatment in C57BL/6 mice. ( E ) Hif1A or <t>Hif2A</t> immunoblotting was performed on protein isolated from whole lung tissue after treatment with vadadustat. Each column represents 1 animal. ( F and G ) Quantification of Hif1a and Hif2a protein after treatment with vadadustat for 3 days. Data are presented as mean ± SD. ( H ) Schematic diagram of WA1 infection (280 PFU) in K18-hACE2 mice treated with vadadustat. ( I ) Kaplan-Meier plots of K18-hACE2 mice with vehicle or vadadustat treatment. P values were calculated with the Mantel-Cox test. ( J ) Blinded histological injury scores of the lungs were quantified as described in the Methods. Data are represented as mean ± SEM. ( K ) Representative H&E staining images of lung tissue from vehicle- and vadadustat-treated mice. Scale bars: 50 μm. ( L ) Schematic diagram of MA10 infection (200 PFU) in BALB/c mice treated with vadadustat. ( M ) Kaplan-Meier plots of BALB/c mice with vehicle or vadadustat treatment. P values were calculated with the Mantel-Cox test. * P < 0.05, ** P < 0.01, *** P < 0.001 by 1-way ANOVA with Dunnett’s multiple-comparison test ( C ) or 2-tailed Student’s t test ( F , G , and J ).
    Primary Antibodies Targeting Hif2a, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primary+antibodies+targeting+hif2a/pmc12288972-172-5-11?v=Novus+Biologicals
    Average 96 stars, based on 1 article reviews
    primary antibodies targeting hif2a - by Bioz Stars, 2026-07
    96/100 stars
    		  Buy from Supplier

    Image Search Results


    ( A ) Schematic diagram of vadadustat treatment in HIF reporter ODD-luc mice. ( B ) Representative ex vivo bioluminescence imaging of luciferase activity using IVIS imager at 2 hours after vadadustat i.p. injection. ( C ) Quantification of bioluminescence intensity at 2 hours and 4 hours after vadadustat i.p. injection. Data are presented as mean ± SD. ( D ) Schematic diagram of vadadustat treatment in C57BL/6 mice. ( E ) Hif1A or Hif2A immunoblotting was performed on protein isolated from whole lung tissue after treatment with vadadustat. Each column represents 1 animal. ( F and G ) Quantification of Hif1a and Hif2a protein after treatment with vadadustat for 3 days. Data are presented as mean ± SD. ( H ) Schematic diagram of WA1 infection (280 PFU) in K18-hACE2 mice treated with vadadustat. ( I ) Kaplan-Meier plots of K18-hACE2 mice with vehicle or vadadustat treatment. P values were calculated with the Mantel-Cox test. ( J ) Blinded histological injury scores of the lungs were quantified as described in the Methods. Data are represented as mean ± SEM. ( K ) Representative H&E staining images of lung tissue from vehicle- and vadadustat-treated mice. Scale bars: 50 μm. ( L ) Schematic diagram of MA10 infection (200 PFU) in BALB/c mice treated with vadadustat. ( M ) Kaplan-Meier plots of BALB/c mice with vehicle or vadadustat treatment. P values were calculated with the Mantel-Cox test. * P < 0.05, ** P < 0.01, *** P < 0.001 by 1-way ANOVA with Dunnett’s multiple-comparison test ( C ) or 2-tailed Student’s t test ( F , G , and J ).

    Journal: JCI Insight

    Article Title: Identification of HIF1A as a therapeutic target during SARS-CoV-2–associated lung injury

    doi: 10.1172/jci.insight.191463

    Figure Lengend Snippet: ( A ) Schematic diagram of vadadustat treatment in HIF reporter ODD-luc mice. ( B ) Representative ex vivo bioluminescence imaging of luciferase activity using IVIS imager at 2 hours after vadadustat i.p. injection. ( C ) Quantification of bioluminescence intensity at 2 hours and 4 hours after vadadustat i.p. injection. Data are presented as mean ± SD. ( D ) Schematic diagram of vadadustat treatment in C57BL/6 mice. ( E ) Hif1A or Hif2A immunoblotting was performed on protein isolated from whole lung tissue after treatment with vadadustat. Each column represents 1 animal. ( F and G ) Quantification of Hif1a and Hif2a protein after treatment with vadadustat for 3 days. Data are presented as mean ± SD. ( H ) Schematic diagram of WA1 infection (280 PFU) in K18-hACE2 mice treated with vadadustat. ( I ) Kaplan-Meier plots of K18-hACE2 mice with vehicle or vadadustat treatment. P values were calculated with the Mantel-Cox test. ( J ) Blinded histological injury scores of the lungs were quantified as described in the Methods. Data are represented as mean ± SEM. ( K ) Representative H&E staining images of lung tissue from vehicle- and vadadustat-treated mice. Scale bars: 50 μm. ( L ) Schematic diagram of MA10 infection (200 PFU) in BALB/c mice treated with vadadustat. ( M ) Kaplan-Meier plots of BALB/c mice with vehicle or vadadustat treatment. P values were calculated with the Mantel-Cox test. * P < 0.05, ** P < 0.01, *** P < 0.001 by 1-way ANOVA with Dunnett’s multiple-comparison test ( C ) or 2-tailed Student’s t test ( F , G , and J ).

    Article Snippet: Membranes were probed with respective primary antibodies targeting Hif2a (NB 100-122, Novus; diluted 1:2000 in PBST), Hif1a (14179, Cell Signaling Technology; diluted 1:2000 in PBST), or α-tubulin (2144, Cell Signaling Technology; diluted 1:2000 in PBST) and incubated overnight at 4°C with gentle swirling.

    Techniques: Ex Vivo, Imaging, Luciferase, Activity Assay, Injection, Western Blot, Isolation, Infection, Staining, Comparison

    ( A ) Mice were inoculated with 3 × 10 4 PFU of the murine-adapted SARS-CoV-2 strain (MA10) via oropharyngeal aspiration, and clinical outcomes were monitored over 7 days. ( B and C ) The survival rate in SARS-CoV-2–infected mice with whole-body deletion of Hif1a ( Hif1a fl/fl UBCCreER) or ( C ) Hif2a-deleted mice ( Hif2a fl/fl UBCCreER) compared to their respective Hif fl/fl litter mates. P values were obtained using the Mantel-Cox test. ( D ) Mice with a specific deletion of Hif1a in alveolar epithelial cells ( Hif1a fl/fl SPCCreER) and their Cre-inducible counterpart (SPCCreER) were infected with 3 × 10 3 PFU of the MA10 strain via oropharyngeal aspiration or mock infected, monitored for clinical outcomes and euthanized on day 4 to harvest BALF and lung tissue. ( E ) Albumin concentration in BALF was measured by ELISA. Data are represented as mean ± SEM. Two-tailed Student’s t test. ( F and G ) Viral load in BALF and lung tissue was detected by plaque assay. Gaussian distribution was assayed using the Shapiro-Wilk test. Unpaired 2-tailed Student’s t test or Mann-Whitney U test was applied to parametric or nonparametric data, respectively. ( H ) The lungs of infected SPCCreER and Hif1a fl/fl SPCCreER mice 4 days after infection were collected, fixed, and paraffin embedded. H&E staining was performed, and images were taken at ×10 magnification ( n = 5 or 8, respectively; representative images are shown). Scale bars: 200 μm. ( I ) The lung injury score was performed blindly. In the bar-and-whisker plots, the bounds of the boxes represent the 25%–75% interquartile range, the lines within the boxes represent the median, the whiskers represent data min/max, and there are no outlying values. Two-tailed Student’s t test. ( J ) Inflammatory molecules were measured using a multiplex array in the BALF from SPCCreER and Hif1a fl/fl SPCCreER SARS-CoV-2– or mock-infected mice. Volcano plot resulting from an unpaired 2-tailed Student’s t test with Welch’s correction comparing both groups. Molecules that were highly differentially secreted are emphasized in red. The column graphs represent individual results for IL-6, G-CSF, and IP-10 ( n = 10–12). Unpaired 2-tailed Student’s t tests with Welch’s correction or Mann-Whitney U test was applied to parametric or nonparametric data. Normality was established using the Shapiro-Wilk test. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Journal: JCI Insight

    Article Title: Identification of HIF1A as a therapeutic target during SARS-CoV-2–associated lung injury

    doi: 10.1172/jci.insight.191463

    Figure Lengend Snippet: ( A ) Mice were inoculated with 3 × 10 4 PFU of the murine-adapted SARS-CoV-2 strain (MA10) via oropharyngeal aspiration, and clinical outcomes were monitored over 7 days. ( B and C ) The survival rate in SARS-CoV-2–infected mice with whole-body deletion of Hif1a ( Hif1a fl/fl UBCCreER) or ( C ) Hif2a-deleted mice ( Hif2a fl/fl UBCCreER) compared to their respective Hif fl/fl litter mates. P values were obtained using the Mantel-Cox test. ( D ) Mice with a specific deletion of Hif1a in alveolar epithelial cells ( Hif1a fl/fl SPCCreER) and their Cre-inducible counterpart (SPCCreER) were infected with 3 × 10 3 PFU of the MA10 strain via oropharyngeal aspiration or mock infected, monitored for clinical outcomes and euthanized on day 4 to harvest BALF and lung tissue. ( E ) Albumin concentration in BALF was measured by ELISA. Data are represented as mean ± SEM. Two-tailed Student’s t test. ( F and G ) Viral load in BALF and lung tissue was detected by plaque assay. Gaussian distribution was assayed using the Shapiro-Wilk test. Unpaired 2-tailed Student’s t test or Mann-Whitney U test was applied to parametric or nonparametric data, respectively. ( H ) The lungs of infected SPCCreER and Hif1a fl/fl SPCCreER mice 4 days after infection were collected, fixed, and paraffin embedded. H&E staining was performed, and images were taken at ×10 magnification ( n = 5 or 8, respectively; representative images are shown). Scale bars: 200 μm. ( I ) The lung injury score was performed blindly. In the bar-and-whisker plots, the bounds of the boxes represent the 25%–75% interquartile range, the lines within the boxes represent the median, the whiskers represent data min/max, and there are no outlying values. Two-tailed Student’s t test. ( J ) Inflammatory molecules were measured using a multiplex array in the BALF from SPCCreER and Hif1a fl/fl SPCCreER SARS-CoV-2– or mock-infected mice. Volcano plot resulting from an unpaired 2-tailed Student’s t test with Welch’s correction comparing both groups. Molecules that were highly differentially secreted are emphasized in red. The column graphs represent individual results for IL-6, G-CSF, and IP-10 ( n = 10–12). Unpaired 2-tailed Student’s t tests with Welch’s correction or Mann-Whitney U test was applied to parametric or nonparametric data. Normality was established using the Shapiro-Wilk test. * P < 0.05, ** P < 0.01, *** P < 0.001.

    Article Snippet: Membranes were probed with respective primary antibodies targeting Hif2a (NB 100-122, Novus; diluted 1:2000 in PBST), Hif1a (14179, Cell Signaling Technology; diluted 1:2000 in PBST), or α-tubulin (2144, Cell Signaling Technology; diluted 1:2000 in PBST) and incubated overnight at 4°C with gentle swirling.

    Techniques: Infection, Concentration Assay, Enzyme-linked Immunosorbent Assay, Two Tailed Test, Plaque Assay, MANN-WHITNEY, Staining, Whisker Assay, Multiplex Assay